Vascular flora of Kenya, based on the Flora of Tropical East Africa

Abstract Kenya, an African country with major higher plant diversity, has a corresponding diversity of plant associations, because of the wide geographic distribution, diverse climatic conditions and soil types. In this article, all vascular plants of Kenya were counted based on the completed "Flora of Tropical East Africa (FTEA)", and all families and genera were revised using recent molecular systematics research, forming a "Synoptic List of Families and Genera of Kenyan Vascular Plants (SLFGKVP)". In total, there are 225 families, 1538 genera and 6293 indigenous species and and 62 families, 302 genera and 588 exotic species in Kenya. The Fabaceae with 98 genera and 576 Species is the largest family. Two of the seven plant distribution regions of Kenya, K4 and K7 are the most species-richest areas with regard to both total and endemic species, with 3375 and 3191 total species and 174 and 185 endemic species in K4 and K7 respectively. While, K3 and K5 have the highest density of both total and endemic species. K1 has the lowest density of total species, and K2 has the lowest density of endemic species.


Introduction
The Republic of Kenya ( Fig. 1) lies in the equatorial zone of eastern part of Africa continent, between latitudes 4°N and 4°S and between longitudes 34°E and 42°E. The diverse vegetation types of Kenya include numerous indigenous plant species, many of which are endemic, such as Dendrosenecio keniensis (Asteraceae) in K4 (Mt. Kenya), Impatiens fischeri (Balsaminaceae) in K3 and K4, Habenaria keniensis (Orchidaceae) in K3, K4 and K5, and the recently published species Sedum keniense (Crassulaceae) in K4 (Mt. Kenya) (Zhou et al. 2016a). Over the years, botanists from all over the world have studied Kenyan plants and accumulated a series of monographs. In 1961, Dale I.R. and Greenway P.J. published "Kenya Trees and Shrubs", which recorded about 1000 trees and shrubs of Kenya (Dale and Greenway 1961), and Beentje H.J. republished this book in 1994 reviewing some species and adding lianas (Beentje 1994). In 1974, Agnew A.D.Q. published "Upland Kenya Wild Flowers" andrepublished it in 1994, recording over 3000 herbs and ferns of Kenyan upland areas; the third edition of which was published in 2013, with the addition of grasses and sedges (Agnew 1974, 2013, Agnew and Agnew 1994. Other books on Kenya plants include, "The wild flowers of Kenya" (Blundell, 1982), "An illustrated manual of Kenya Grasses" (Ibrahim and Kabuye 1987), "The beautiful plants of Kenya" (Karmali 1988), "Orchids of Kenya" (Stewart 2003), "Useful trees and shrubs for Kenya" (Maundu and Tengnas 2005), "Common plants of Kenya" (Wang et al. 2016), and some other books with a broader coverage of whole east Africa, such as "Collins guide to the wild flowers of East Africa" (Blundell 1987), "Field Guide to Acacias of East Africa" (Dharani 2006), "Medicinal Plants of East Africa, 3 rd Edition" (Kokwaro 2009), "Medicinal Plants of East Africa-An Illustrated Guide" (Dharani and Yenesew 2010), "Field Guide to Common Trees and Shrubs of East Africa, 2 nd Edition" (Dharani 2011). "Flora of Tropical Africa" was the first flora record of Kenya plants, which included 13000 plants of tropical Africa and divided into 10 volumes (Daniel et al. 1868(Daniel et al. -1933. "Flora of Tropical East Africa (FTEA)" covers plants from Uganda, Kenya and Tanzania, which has been edited several times since 1952 and completed in 2012. To date, FTEA records c.125000 plant species from the three countries. Shortcomings of FTEA on Kenya plants are that: i) taxa are still under-represented; ii) New families, genera and taxa have not been reviewed and recorded; iii) Families have not been arranged using the recent available botanical systems.
Plant systematics has been one of the hottest research areas in botanical studies (Singh 2010, Simpson 2010. In the early years, some plants systems were published based on morphological characteristics, such as Ching"s system on ferns (Ching 1978a, b), Cheng"s system about gymnosperms (Cheng and Fu 1978), and Takhtanjan"s system, Cronquist"s system and Engler"s system about angiosperms (Yang et al. 2004, Liu et al. 2015. Lycophytes had been separated from ferns according to recent molecular systematics evidences, and was positioned as the sister group to Euphyllophytes (including Spermatophytes and Monilophytes) (Pryer et al. 2004, Liu et al. 2009). Smith et al. (2006) proposed a new classification system on Monilophytes, dividing ferns into 4 classes, 10 orders and 37 families, and later some new families were established and new classification system was proposed (Christenhusz et al. 2011b, Liu et al. 2013, Zhang and Zhang 2015. Recently, the Pteridophyte Phylogeny Group proposed a community-derived classification on extant lycophytes and ferns, including 2 classes, 14 orders and 51 families (PPG I 2016). Based on branch taxonomy and molecular systematics results, the Angiosperm Phylogeny Group (APG) proposed a new classification, refined over four publications (APG 1998, APG II 2003, APG III 2009, APG IV 2016. Compared with the APG III system, the APG IV system proposed two additional informal major clades, namely superrosids and superasterids, and recognized 5 new orders, namely Boraginales, Dilleniales, Icacinales, Metteniusales and Vahliales, resulting in a total of 64 orders and 416 families of Angiosperms (APG IV 2016). This article summarizes families and genera of Kenya plants based on FTEA, and combined with other recent molecular systematic research. Our goal was to answer the following questions: i) What is the total number of plant species found in Kenya? ii) Which families and genera are the largest? iii) Which part in Kenya contains the highest plant species richness, and iv) What measures can be carried out so as to protect and conserve the Kenyan plants?

Data and methods
All information is sourced from the FTEA, since it has recorded over 12500 vascular plants of Uganda (U1-U4), Kenya (K1-K7) and Tanzania (T1-T8), including some planted and naturalized species. Each family has been published in one volume, although some big families with 2-4 volumes also have been done, such as Leguminosae (4 volumes), Poaceae (3 volumes), Rubiaceae (3 volumes) and Compositae (3 volumes). Except for some exotic plants, FTEA has described each species, the distribution regions, and characteristic habitat. and recorded the voucher specimens.
Here, we conducted a census of all Kenyan species recorded by FTEA, gathering distribution information on endemic plants, exotic plants and naturalized species. We then reviewed the families and genera of all the species based on recent systematic research, creating a "Synoptic List of Families and Genera of Kenyan Vascular Plants (SLFGKVP)" (see Supplementary material 1). Families of Lycophytes and monilophytes are revised by PPG I system (PPG I 2016), families of gymnosperms are revised the system advocated by Christenhusz et al. (2011a), and families of angiosperms are revised by APG IV system (APG IV 2016). We analyzed the largest families and genera, as well as the number of endemic and exotic species of Kenya plants. We also compared total and endemic species number, and species density among different regions (K1-K7) of Kenya.

Families, genera and species of indigenous plants
A total of 6293 Kenyan indigenous vascular plants were recorded by FTEA, representing 225 families and 1538 genera, of which 95.5% are angiosperms, 4.0% are monilophytes, 0.37% are Lycophytes and a very small percentage are gymnosperms. Three families, 5 genera and 23 species belong to lycophytes; 27 families, 87 genera and 252 species belong to monilophytes; 3 families, 3 genera and 5 species belong to gymnosperms; 192 families, 1443 genera and 6013 species belong to angiosperms. Based on new systematic systems, the families number of SLFGKVP decrease to 224, including 3 families of Lycophytes, 28 families of monilophytes, 3 families of gymnosperms and 190 families of angiosperms. Because we fully accepted FTEA"s treatment on synonyms, the genera and species number kept the same.
The top three species-rich families of Kenyan indigenous vascular plants are Fabaceae, Poaceae and Asteraceae, which contain 576, 565 and 403 species, respectively. The top three species-rich genera are Euphorbia (Euphorbiaceae), Cyperus (Cyperaceae) and Crotalaria (Fabaceae), which contain 95, 94 and 93 species, respectively. The most species-rich family and genus of monilophytes are Aspleniaceae and Asplenium with 51 species (Table 1).

Endemism
There is no endemic family in Kenya, but there is one endemic genus, which is Dibrachionostylus, a monotypic genus of Rubiaceae restricted to K4. The genus has only one species, D. kaessneri and is closely related to Hedythyrsus and Agathisanthemum (Verdcourt 1976).
In total, 467 endemic species including unnamed ones were recorded in FTEA. The endemic taxa of Lycophytes are mainly in Isoetes (Isoetaceae), 3 species out of 4 in this genus in Kenya being endemic. Lycopodium aberdaricum is also an endemic lycophyte only found in K3 and K4. There are only 4 endemic species in monilophytes and 3 belong to Marsileaceae. Another unnamed species of Asplenium (Aspleniaceae) recorded as endemic. There are no endemic gymnosperms. However, in angiosperms, 459 species are endemic, with the top contributing families for endemics the Euphorbiaceae (50 endemic species), Fabaceae (40 endemic species) and Acanthaceae (33 endemic species) ( Table 2). There are 12 families with more than 10 endemic species, with the endemic ones in Asphodelaceae contributing 38.33% of the total species in that family (Table 2).

Exotic plants of Kenya
A total number of 588 exotic plants including 212 naturalized species of Kenya were recorded in FTEA, which belong to 62 families and 302 genera. The top 10 exotic families and their species number are shown in Table 3. Myrtaceae, Fabaceae, Asteraceae and Solanaceae are the 4 largest families which have more than 40 exotic species, and Eucalyptus (Myrtaceae) is the largest exotic genus with 99 exotic species.

Distribution patterns
The total number of indigenous and endemic species within each distribution region was counted (Fig. 2). Because species in gymnosperms are few, there is no significant uniformity (Fig. 2c). The results of Lycophytes (Fig. 2a), monilophytes (Fig. 2b), angiosperms (Fig. 2d) and the total species ( Fig. 2e; Table 4) had similar patterns among seven regions and showed that K4 and K7 are the two most species-rich regions with regard to both total and endemic species. If the areas of different regions were taken into account, K3 and K5 have the highest density of total vascular plants, with ca. 776 species/10000 km 2 in K3 and ca. 768 species/10000 km 2 in K5. K3 and K7 have the     highest density of endemic vascular plants, with ca. 24 species/10000 km 2 in K3 and ca. 27 species/10000 km 2 in K7. In contrast, K1 has the lowest density of total species (ca. 86 species/10000 km 2 ) and K2 has the lowest density of endemic species (ca. 4 species/10000 km 2 ) (Table 4).

Discussion
The diversity  The top three species-rich families of Kenya are Fabaceae, Poaceae and Asteraceae, which are also the largest families in the world, having 745 genera/19560 species, 707 genera/11337 species and 1620 genera/23600 species respectively (Stevens 2012). The proportion of Kenyan plants within the top total species in the world in these families is very small, though the endemism in them is relatively high (Table 1, 2).
Totally, 3375 indigenous vascular plants have been found in K4 ( Fig. 2e; Table 4). The main vegetation types of K4 is Afromontane rain forest, Afromontane moist transitional forest, Dry Combretum wooded grassland and Acacia-Commiphora deciduous bushlandthicket (VECEA team 2015). The two most species-rich areas of K4 are Mt. Kenya and its neighboring Aberdare Ranges (Fig. 1). Montane forest vegetation as well as the unique Afroalpine vegetation also have high plant diversity and special species (Mittermeier et al. 2004, 2011, Gehrke and Linder 2014, such as Vitex keniensis (Lamiaceae), Impatiens fischeri (Balsaminaceae), Cyphostemma grahamii (Vataceae), Ranunculus keniensis (Ranunculaceae), Dendrosenecio keniensis (Asteraceae), Lobelia deckenii (Campanulaceae) (Young 1985, Bussmann 1995. There is also an endemic genus, Dibrachionostylus (Rubiaceae), recorded in K4 (Verdcourt 1976). There are at least 774 species, subspecies and varieties of vascular plants within the Aberdare Ranges, belonging to 213 genera, 128 families (Schmitt 1991). About 880 species, subspecies and varieties belonging to 479 genera and 146 families from Mt. Kenya below 3200 m altitude were recorded by Beentje (1991) and Bussman (1994), and a recent research results showed that more than 1500 vascular species are growing in this area, representing over 20% of total plants in Kenya (Zhou 2017). The Coastal Forests of Eastern Africa is one of the two diversity hotpots in east Africa, which is expanded to Somalia and Mozambique (Mittermeier et al. 2004(Mittermeier et al. , 2011. In our study, we recognise 3191 indigenous plants and 185 endemic plants in K7 ( Fig. 2e; Table 4). K7 is located in the coastal areas of Kenya (Fig. 1) and has highest density of endemic plants with ca. 27 species/10000 km 2 , though the density of total indigenous plants is not very high with ca. 471 species/1000 km 2 (Table 4). The two main vegetation types of K7 are Acacia-Commiphora deciduous bush land-thicket and Coastal mosaic (VECEA team 2015). A number of tropical coastal plants are living here, such as Ceriops tagal and Bruguiera gymnorhiza (Rhizophoraceae), Sonneratia alba (Lythraceae), Xylocarpus granatum (Meliaceae) and Lumnitzera racemosa (Combretaceae) (Dharani 2011).

Flora of Kenya
To date, there is no known regional flora within Kenya. "Flora of Tropical Africa", "Flora of East Tropical Africa" and other floras all have a long publication history. However, some information in them is not accurate, and a lot of species are still unnamed. In recent years, many new species, new records and new taxonomic treatments have been found and proposed. A series of surveys on plant resources at some biodiversity hotspots of Kenya have been carried out. Numerous plant checklists covering different regions of Kenya have been published, such as Mt. Elgon (Tweedie 1976), Mt. Nyiru (Bytebier and Bussmann 2000), Taita hills (Thijs et al. 2013), Nandi Forests (Girma et al. 2015). Therefore, an up-to-date and comprehensive flora (i.e. "Flora of Kenya") is urgently needed, as this will further promote further botanical studies and help action conservation measures to protect the plant diversity in Kenya. At present, Chinese researchers are actively involved in the preparation of a new Flora of Kenya, and contributing their skills to help the Kenyan people determine exactly what plants live in Kenya, to enable better protection for them, and to develop and utilize where practical the extremely high diversity of Kenyan plants.

Conservation of plant diversity in Kenya
Due to human disturbance and destruction, exotic plant invasion, climate change, deterioration of ecological environment and other factors, the plant diversity of Kenya is also facing severe pressures (FAO 1981, Barnes 1990, Bussmann 1996, Gathaara 1999. For example, at the foot of Mt. Kenya, a large area of the original forest was cut down for plantations of Cupressus lusitanica, Pinus patula and Eucalyptus spp. for timber and also some cultivations of crops such as banana (Musa spp.), potato (Solanum tuberosum) and maize (Zea mays) (Bussmann 1996, Gathaara 1999. K3, K4, K5 and K7 have high plant diversity and high density of total and endemic plants. Consideration of better protection measures of a representation of these distribution regions is urgently needed. also thank Can Dai from School of Resources and Environmental Science, Hubei University, for improving the use of English in the manuscript. Thanks also due to P. de Lange, E. Farris and another anonymous reviewer for their valuable comments on the manuscript. This work was supported by Sino-Africa Joint Research Centre (Y323771W07 and SAJC201322) and National Natural Science Foundation of China (31361140360).